Harnessing the power of CRISPR to program next generation diagnostics bringing answers to people who need them the most.

What is SHERLOCK™?

SHERLOCK is an evolution of CRISPR technology, which others use to make precise edits in genetic code.

As a diagnostic tool, SHERLOCK can detect the unique genetic fingerprints encoded in virtually any DNA or RNA sequence in any organism or pathogen.

Accelerating CRISPR diagnostics

SHERLOCK utilizes CRISPR as a method for “smart amplicon detection” and can be adapted for use in a single cartridge as part of a simple point-of-need system or deployed as a single-use disposable in low-resource settings.

The adaptability of SHERLOCK, coupled with its ability to precisely identify nucleic acid sequences, makes it a diagnostic with potential for broad global reach.

Rapid assay development and deployment

Easily portable to existing instrument platforms

Adapted for high and low throughput systems

Real-time multiplexing with expanded enzyme functionality

How SHERLOCK Works

Developed by our founders and licensed exclusively from the Broad Institute, SHERLOCK is a method for single molecule detection of nucleic acid targets.

Specific High-Sensitivity Enzymatic Reporter UnLOCKing

It works by programming special CRISPR-Cas enzymes to detect the presence of a specific nucleic acid signature in a sample through 'smart amplicon detection.' When the signature is detected, the CRISPR-Cas enzyme is activated to generate a robust signal. This signal can be adapted to work on a simple paper strip test, in laboratory equipment, or to provide an electrochemical readout that can be read with a mobile phone.