Harnessing the power of CRISPR to program next generation diagnostics bringing answers to people who need them the most.

What is SHERLOCK™?

SHERLOCK is an evolution of CRISPR technology, which others use to make precise edits in genetic code.

As a diagnostic tool, SHERLOCK can detect the unique genetic fingerprints encoded in virtually any DNA or RNA sequence in any organism or pathogen.

Accelerating CRISPR diagnostics

SHERLOCK utilizes CRISPR as a method for “smart amplicon detection” and can be adapted for use in a single cartridge as part of a simple point-of-need system or deployed as a single-use disposable in low-resource settings.

The adaptability of SHERLOCK, coupled with its ability to precisely identify nucleic acid sequences, makes it a diagnostic with potential for broad global reach.

Rapid assay development and deployment
Easily portable to existing instrument platforms
Adapted for high and low throughput systems
Real-time multiplexing with expanded enzyme functionality

How SHERLOCK Works

Developed by our founders and licensed exclusively from the Broad Institute, SHERLOCK is a method for single molecule detection of nucleic acid targets.
Specific High-Sensitivity Enzymatic Reporter UnLOCKing
It works by programming special CRISPR-Cas enzymes to detect the presence of a specific nucleic acid signature in a sample through 'smart amplicon detection.' When the signature is detected, the CRISPR-Cas enzyme is activated to generate a robust signal. This signal can be adapted to work on a simple paper strip test, in laboratory equipment, or to provide an electrochemical readout that can be read with a mobile phone.
Sample Collection
Access DNA or RNA from blood, saliva, urine, or swabs
Amplification, CRISPR Detection, and Readout
Generate large amounts of a target of interest
Simultaneous target recognition by Cas enzyme activates collateral activity
Collateral cleavage of nucleic acid reporter gives fluorescent readout
Listen to our CTO William Blake discuss our SHERLOCK platform

Contact us to learn more about how we are engineering biology to test anywhere, everywhere.

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